A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21^Waf1/CIP1 Expression

Figure 2

Characterization of dsP21-397-mediated upregulation of p21 expression. (a) and (b) Time-course of dsP21-397-mediated upregulation of p21 expression. T24 and EJ cells were transfected with dsP21-397 at 50 nM. Cells were subjected to real-time PCR at the indicated time points. and compared to 0 h; and compared to 24 h; compared to 48 h. (c) Expression of p21 protein was assessed by western blotting analysis. GAPDH served as a loading control. Cells were transfected with 50 nM dsP21-397 for the indicated periods of time. (d) and (e) Dose-dependent upregulation of p21 mRNA following transfection with dsP21-397 or miR-1180-5p at the indicated concentrations. Cells were subjected to real-time PCR at 72 h. GAPDH served as a loading control. , , and compared to corresponding 0 nM; , , and compared to corresponding 25 nM; and compared to corresponding 50 nM.

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