A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21^Waf1/CIP1 Expression

Figure 3

dsP21-397 inhibits cyclins and CDKs expression in BC cell lines. T24 and EJ cells were transfected with 50 nM of the indicated dsRNAs for 72 h. mock sample was transfected in the absence of dsRNA. (a) Expression of Cyclin D1, CDK4/6 and Cyclin A2 protein in BC cells was detected by Western blot. α-tublin served as a loading control. (b) and (c) Densitometric analysis quantification of Cyclin D1, CDK4/6, and Cyclin A2 proteins expression in T24 and EJ cells. , , and compared to mock group; , , and compared to dsControl group.