Research Article
A New Double Stranded RNA Suppresses Bladder Cancer Development by Upregulating p21Waf1/CIP1 Expression
Figure 5
dsP21-397 inhibits cyclins and CDKs expression mainly by regulating p21. Cells were transfected with 50 nM of the indicated dsRNAs for 72 h. (a) Expression of p21 was assessed by real-time PCR. GAPDH served as a loading control. compared to dsP21-397 group. (b) Induction of p21 protein expression was detected by Western blot analysis. GAPDH were also detected and served as a loading control. (c) Expression of Cyclin D1, CDK4/6, and Cyclin A2 protein was detected by Western blot. α-tublin served as a loading control. (d) and (e) Densitometric analysis quantification of Cyclin D1, CDK4/6, and Cyclin A2 proteins expression in T24 and EJ cells. and compared to dsControl group; , , and compared to dsP21-397 group.