Role of Genetic Polymorphisms of Deoxycytidine Kinase and Cytidine Deaminase to Predict Risk of Death in Children with Acute Myeloid Leukemia

Figure 1

Identification of the genotypes of CDA and dCK genes. (a) Polymorphism A79C of CDA gene. (A1) CDA gene PCR. M 100 bp DNA ladder; lines 1–4: PCR product prior to digestion 205 bp. (A2) Restriction with Eco RI. M 100 bp DNA ladder; line 1: heterozygous (AC); line 2: variant homozygous (CC); line 3: wild-type homozygous (AA). (b) Polymorphism C360G and C201T of dCK gene. (B1) dCK gene PCR. M 100 bp DNA ladder; lines 1–3: PCR product prior to digestion 583 bp. (B2) Restriction with Kas I. M 100 bp DNA ladder; line 1: variant homozygous (GG); line 2: heterozygous (CG); line 3: wild-type homozygous (CC). (B3) Restriction with Bgl I. M 100 bp DNA ladder; line 1: variant homozygous (TT); line 2: heterozygous (CT); line 3: wild-type homozygous (CC).

(a) Detection of the CDA A79C polymorphism

(b) Detection of the dCK C360G and C201T polymorphisms