Lutein Has a Protective Effect on Hepatotoxicity Induced by Arsenic via Nrf2 Signaling

Figure 2

(a) Real time PCR analysis of treatment of arsenic trioxide (ATO) and/or lutein (LU). Nuclear factor erythroid 2-related factor 2 (Nrf2, molecular weight 173 bp), NAD(P)H dehydrogenase, quinone 1 (Nqo1, molecular weight 112 bp), heme oxygenase-1 (Ho-1, molecular weight 184 bp), and glutathione transferase (Gst, molecular weight 199 bp) mRNA expression levels were measured by real time PCR. (b) Quantitative mRNA analysis of treatment of arsenic trioxide (ATO) and/or lutein (LU). -axis indicates mRNA expression of test marker versus β-actin. Each bar represents the mean ± SD. The gene expression of Nrf2, Nqo1, Ho-1, and Gst of LU treat group was significantly higher than those of control group. The group treated with ATO + LU showed the most prominent mRNA expression of Nrf2 related genes. Significant differences relative to the control and ATO groups are indicated as follows: * versus control group, ; # versus ATO group, .

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