Prohibitin: A Novel Molecular Player in KDEL Receptor Signalling

Figure 6

Intracellular distribution of endogenous KDELR in PHB knock-down cells. (a) KDELR redistributes from the Golgi in PHB-depleted cells. HeLa cells were treated with scrambled siRNAs (Control) or with siRNAs against PHB (PHB siRNA,) for 96 h. The cells were fixed and stained for KDELR (red) and GM130 (marker for Golgi area definition, green); the merged images are also shown. Scale bars, 10 μm. (b) Quantification of KDELR in the Golgi area of HeLa cells treated as in (a). Data are means ± SEM for KDELR immunofluorescence on the Golgi, from three independent experiments, with at least 50 cells quantified per experiment. compared to control cells (-test). (c) PHB knockdown does not affect the expression levels of KDELR. HeLa cells treated as in (a) were lysed, and their proteins analysed by Western blotting using antibodies to KDELR. The levels of PHB knockdown were investigated as a control.

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