Impact of Viral Activators and Epigenetic Regulators on HIV-1 LTRs Containing Naturally Occurring Single Nucleotide Polymorphisms

Figure 4

HIV-1 LAI non-GFP-expressing LTR is not inducible by the combination of TSA and TNF-α above levels seen with TNF-α alone in stably transfected TF-1 and U-937 cell clones. Stably transfected TF-1 cell clones containing the WT, 3T, 5T, or 3T5T LAI LTRs were treated with 100 nM trichostatin A (TSA) in combination with TNF-α (20 ng/mL) for 24 hours, along with untreated clone controls as well as untreated and treated TF-1 cells (a) and U-937 cells (b). After 24 hours, cells were analyzed for GFP expression using flow cytometry. All experiments were completed in triplicate in three independent experiments. Representative histograms show levels of GFP expression of the untreated cells (solid black line) compared to the treated cells (dashed black line), and untreated stably transfected cell clones (solid turquoise line) compared to the treated cell clones (dashed turquoise line). TSA alone did not result in any alterations in gene expression within any of the cell clones examined.

(a)

(b)