In Vitro Behavior of Human Adipose Tissue-Derived Stem Cells on Poly(ε-caprolactone) Film for Bone Tissue Engineering Applications

Figure 10

Representative observations of the PA42-C4 line on PCL film induced in osteogenic medium up to 35 days. ALP+ cells are stained with Fast Red Violet LB (red color) and nuclei with DAPI (fluorescent blue color). Image acquired in brightfield microscopy for ALP+ cells and in epifluorescence for nuclei. Objective 20x.