Research Article
Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli
Table 1
Primer pairs used to specifically amplify target gene coding for virulence factors (1–9 = toxins; 9–15 fimbriae) of E. coli and 16S rRNA (16-17).
| | Target gene coding for virulence factors | Oligonucleotide sequences of primers | Reference |
| | (1) LT | F 5′-ATT TAC GGC GTT ACT ATC CTC-3′
R 5′-TTT TGG TCT CGG TCA GAT ATG-3′ | [25] |
| | (2) Sta | F 5′-TCC GTG AAA CAA CAT GAC GG-3′
R 5′-ATA ACA TCC AGC ACA GGC AG-3′ | [26] |
| | (3) STb | F 5′-GCC TAT GCA TCT ACA CAA TC-3′
R 5′-TGA GAA ATG GAC AAT GTC CG-3′ | [26] |
| | (4) Stx1all | F 5′-CGC TGA ATG TCA TTC GCT CTG C-3′
R 5′-CGT GGT ATA GCT ACT GTC ACC-3′ | [27] |
| | (5) Stx2all | F 5′-CTT CGG TAT CCT ATT CCC GG-3′
R 5′-CTG CTG TGA CAG TGA CAA AAC GC-3′ | [27] |
| | (6) Stx2e | F 5-ATG AAG AAG ATG TTT ATA GCG-3′
R 5′-TCA GTT AAA CTT CAC CTG GGC-3′ | [25] |
| | (7) EAST1 | F 5′-CCA TCA ACA CAG TAT ATC CGA-3′
R 5′-GGT CGC GAG TGA CGG CTT TGT-3′ | [28] |
| | (8) eae | F 5′-GGA ACG GCA GAG GTT AAT CTGCAG-3′
R 5′-GGC GCT CAT CAT AGT CTTTC-3′ | [27] |
| | (9) hlyA | F 5′-AGCTGCAAGTGCGGGTCTG-3′
R 5′-TACGGGTTATGCCTGCAAGTTCAC-3′ | [29] |
| | (10) F4 (K88) | F 5′-GCT GCA TCT GCT GCA TCT GGTATG G-3′
R 5′-CCA CTG AGT GCT GGTAGT TAC AGC C-3′ | [30] |
| | (11) F5 (K99) | F 5′-TGC GAC TAC CAA TGC TTC TG-3′
R 5′-TAT CCA CCA TTA GAC GGA GC-3′ | [26] |
| | (12) F6 (P987) | F 5′-TCT GCT CTT AAA GCT ACT GG-3′
R 5′-AAC TCC ACC GTT TGT ATC AG-3′ | [25] |
| | (13) F17 | F 5′-GGG CTG ACA GAG GAG GTG GGGC-3′
R 5′-CCC GGC GAC AAC TTC ATCACC GG-3′ | [30] |
| | (14) F18 | F 5′-GTG AAA AGA CTA GTG TTT ATT TC-3′
R 5′-CTT GTA AGT AAC CGC GTA AGC-3′ | [31] |
| | (15) F41 | F 5′-GAG GGA CTT TCA TCT TTT AG-3′
R 5′-AGT CCA TTC CAT TTA TAG GC-3′ | [26] |
| | (16) E16SI | F 5′-CCCCCTGGACGAAGACTCAC-3′
R 5′-ACCGCTGGCAACAAAGGATA -3′ | [29] |
| | (17) E16SII | F 5′-AGAGTTTGATGGCTCAG-3′
R 5′-GGACTACCAGGGTATCTAAT-3′ | [31] |
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