Neem (Azadirachta indica A. Juss) Oil to Tackle Enteropathogenic Escherichia coli

Table 2

List of primer pairs’ amplification products, coordinates, and accession numbers.

Target gene coding for virulence factors	Amplicon (bp)	Primer coordinates	Accession number
(1) LT	281	27–47, 287–307	S60731
(2) STa	244	267–286, 492–510	M58746
(3) STb	279	515–534, 773–793	AY028790
(4) Stx1all	302	113–134, 394–414	M17358
(5) Stx2all	516	50–69, 543–565	M59432
(6) Stx2e	264	1176–1196, 1419–1439	M36727
(7) EAST1	111	2–24, 94–114	S81691
(8) eae	775	1441–1460, 2193–2215	AF022236
(9) hylA	569	867-885, 1435–1412	X79839
(10) F4 (K88)	792	31–54, 798–822	M29374
(11) F5 (K99)	450	45–64, 475–494	M35282
(12) F6 (P987)	333	193–212, 506–525	M35257
(13) F17	411	289–310, 677–699	AF055313
(14) F18	510	1–23, 490–510	M61713
(15) F41	431	154–173, 565–584	X14354
(16) E16SI	401	1628-170, 2063–2082	AB035924
(17) E16SII	798	8-27, 798–805	J01859