DNA Methylation and Histone Modifications Are the Molecular Lock in Lentivirally Transduced Hematopoietic Progenitor Cells

Figure 3

Relative transgene copy number. The extracted genomic DNA from the transduced cells at day 2 and 7 after transduction was subjected to PCR to amplify the transgene region of the sample. Lane 1: 1 kb ladder; Lanes 2–4: PCR with primers specific for HPRT gene; Lane 2: Untransduced genomic DNA (positive control for PCR); Lane 3: transduced genomic DNA extracted at day 2 after transduction (positive control for relative transgene copy number); Lane 4: transduced genomic DNA extracted at day 7 after transduction (positive control for relative transgene copy number); Lane 5: PCR reaction without DNA (negative control); Lane 6–9: PCR with primers specific to transgene; Lane 6: untransduced genomic DNA; Lane 7: transduced genomic DNA extracted at day 2 after transduction; Lane 8: transduced genomic DNA extracted at day 7 after transduction; Lane 9: PCR reaction without DNA (negative control for PCR).