Lunasin Inhibits Cell Proliferation via Apoptosis and Reduces the Production of Proinflammatory Cytokines in Cultured Rheumatoid Arthritis Synovial Fibroblasts

Figure 2

Effect of lunasin on the cell cycle of synovial fibroblasts and the expression of the proteins related to G0/G1 cell cycle arrest. (a) Cell cycle progression of synovial fibroblasts was examined by flow cytometry. Synovial fibroblasts were challenged with lunasin at concentrations of 0, 10, 50, 100, and 200 μM for 48 h, respectively. After treatments, the cells were collected and subjected to the analysis of cell cycle distribution by flow cytometry. (b) Quantification of the cell cycle data. Data were expressed as mean ± SD for three independent experiments by GraphPad Prism Software Version 5.0. was considered a significant difference when compared with the control. (c) Synovial fibroblasts were treated with lunasin at the concentrations of 0 and 200 μM for 48 h. The cell samples were harvested and the expression of cell cycle-relevant proteins was evaluated.

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