Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro

Figure 2

ATP content and mitochondrial membrane potential of intact cells. (a) Cellular ATP content of HepG2 was determined after 24 hours exposure with 1 μg/mL LPS (), control (), valinomycin (2.5 μg/mL) for 24 h (), and valinomycin for 20 minutes (). Kruskal-Wallis . (b) Mitochondrial membrane potential measured by the changes in the 590/530 JC-1 emitted fluorescence. Control: , LPS: . Valinomycin (2.5 μg/mL; incubation for 20 minutes) served as a positive control, to depolarize mitochondrial membrane potential (). Data are represented as box and whiskers (Tukey). Groups were compared using Mann-Whitney test (two groups) or Kruskal-Wallis test followed by Dunn’s correction of multiple comparisons (three groups). (c: control; LPS: lipopolysaccharide; val: valinomycin.)

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