Dose Response of Endotoxin on Hepatocyte and Muscle Mitochondrial Respiration In Vitro

Table 2

Time course of 1 g/mL LPS on oxygen consumption and cell viability of isolated and permeabilized human hepatocytes. 8 hours of incubation with 1 g/mL LPS reduces complex II-dependent respiration compared to control (^∗). For cellular oxygen consumption, all values are expressed as pmol O₂/(sec ∗ Mill. Cells). A Wilcoxon test for non-parametric paired data was used to compare the control group with the LPS group at each time point. = 8 for 4 and 16 hours, = 7 for 8 hours in LPS treated and control cells.

Primary hepatocytes permeabilized		Control	1 g LPS/mL
Complex I	4 h	252 (91–351)	287 (116–337)	8	0.547
	8 h	248 (93–340)	198 (88–293)	7	0.156
	16 h	191 (112–368)	172 (113–329)	8	0.641
Complex II	4 h	411 (198–474)	342 (201–510)	8	0.844
	8 h	454 (242–523)	(167–511)	7	0.016
	16 h	317 (239–530)	274 (241–516)	8	0.742
Complex IV	4 h	242 (192–308)	256 (146–346)	8	0.641
	8 h	257 (167–350)	203 (143–383)	7	0.375
	16 h	287 (208–362)	308 (179–388)	8	0.844
Viability (%)	control	98 (93–99)		5
	4 h		91 (83–95)	5
	8 h		99 (99-100)	5
	16 h		97 (93–99)	5