Research Article
Efficient and Specific Detection of Salmonella in Food Samples Using a stn-Based Loop-Mediated Isothermal Amplification Method
Table 3
Optimization of LAMP assay for Salmonella detection by targeting the stn gene.
| Conditions | The tested range | Difference | Good yield | Selected conditiona |
| Temperature | 60–65°C | 1°C | 64–66°C | 65°C | Time | 0–60 min | 5 min | 15–60 min | 25 min | Loop primers | 0.5–1.0 M | 0.1 M | 0.8–1.0 M | 0.8 M | Outer primers | 0.1–0.5 M | 0.1 M | 0.2–0.5 M | 0.2 M | MgSO4 | 4–8 mM | 0.5 mM | 6–7.5 mM | 7 mM | dNTPs | 0.8–2.4 mM | 0.2 mM | 1.2–1.8 mM | 1.6 mM | Betaine | 0.5–1.5 M | 0.1 M | 0.6–1.5 M | 1.0 M | Bst DNA polymerase | 4–12 units | 1 unit | 8–12 units | 8 units |
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The final concentrations of reagents or temperature and times used in LAMP analysis. The concentration of FIP and BIP primers are fixed at 1.6 M.
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