Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

Figure 7

Influence of altered gravity during sounding rocket flight on potential reference genes. GAPDH, HMBS, RPLP0, TBP, and YWHAZ (a), ALB and B4GALT6 (b) RNA expression levels after launch and acceleration (BL, dark gray) and μg (black) conditions of TEXUS-49. Hardware ground controls (H/W, striped) are shown for each experimental group. RNA levels are displayed as fluorescence intensities. GAPDH, HMBS, RPLP0, YWHAZ, ALB, and B4GALT6 show no significant change in RNA levels upon altered gravity, while TBP reacts sensitively to all g conditions. Mean values of at least three measurements with standard deviations are shown. .

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