Regulation of Osteoblast Differentiation by Acid-Etched and/or Grit-Blasted Titanium Substrate Topography Is Enhanced by 1,25(OH)₂D₃ in a Sex-Dependent Manner

Figure 4

Effect of Ti surface topography with or without 1α,25(OH)₂D₃ on osteoblast differentiation of rat calvarial osteoblasts. Male and female calvarial osteoblasts were cultured on TCPS or Ti disks. At confluence, cells were treated for 24 hours with 10⁻⁸M 1α,25(OH)₂D₃. Cell number (a), alkaline phosphatase specific activity (b), and osteocalcin levels (c) were measured. Data are displayed as treatment/control of cells on Ti surfaces to cells on TCPS. versus TCPS; versus A surface; versus GB; , female versus male; versus 0 M 1α,25(OH)₂D₃.

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