miR-193b Modulates Resistance to Doxorubicin in Human Breast Cancer Cells by Downregulating MCL-1

Figure 3

miR-193b directly targets MCL-1 and suppresses its expression in MCF-7/DOXR cells. (a) MCF-7/DOXR cells were transfected with miR-193b mimic or NCO incubating for 24 h, and then the mRNA expression and protein expression of MCL-1 were detected by qPCR analysis and western blot, respectively, . (b) Effect of miR-193b on MCL-1 was assessed with the dual-luciferase reporter system. The miR-193b mimic, pRL-TK, and pMIR reporter containing wild- or mutant-type 3′-UTR of MCL-1 gene were cotransfected into the MCF-7/DOXR cells, .

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