Current and New Approaches in GMO Detection: Challenges and Solutions

Table 4

Representative examples illustrating multiplex PCR CGE methods targeting GMO.

Multiplexing	Methods	Targets	References
Tetraplex	Taxon-specific	Zein and LEC	[50]
	Element-specific	p35S and tNOS
Tetraplex	Taxon-specific	SAD1	[51]
	Element-specific	Cry1Ac, p35S, and tNOS
Pentaplex	Taxon-specific	ADH	[52]
	Event-specific	Bt11, GA21, MON810, and NK603
Hexaplex	Taxon-specific	acp1	[53]
	Event-specific	Bollgard, Bollgard II, RR, 3006-210-23, and 281-24-231
Hexaplex	Taxon-specific	HMG	[54, 55]
	Event-specific	DAS-59122-7, LY038, MON88017, MIR604, and 3272
Octaplex	Event-specific	Bt11, Bt176, Huanong No. 1, GTS40-3-2, T25, MON88913, MON1445, and MIR604	[56]
Octaplex	Taxon-specific	LEC and ssIIb
	Element-specific	pFMV and tNOS	[56]
	Event-specific	TC1507, MON531, NK603, and GA21
Octaplex	Taxon-specific	SAD1
	Element-specific	bar, chy, pAct, CP4-EPSPS, and Cry1Ab	[56]
	Event-specific	GT73 and OXY235
Nonaplex	Taxon-specific	HMG	[57, 58]
	Event-specific	T25, GA21, TC1507, MON863, MON810, NK603, Bt176, and Bt11

acp1 (acyl carrier protein 1 gene from cotton); ADH (alcohol dehydrogenase I gene from maize); bar (phosphinothricin-N-acetyltransferases gene from Streptomyces hygroscopicus); Chy (chymopapain gene from papaya); CP4-EPSPS (5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain); Cry (gene encoding the Bacillus thuringiensis δ-endotoxin); HMG (major high-mobility group protein gene from maize); LEC (lectin gene from soybean); p35S (promoter of the 35 S cauliflower mosaic virus); pAct (promoter region of rice actin gene); pFMV (promoter of the figwort mosaic virus); SAD1 (stearoyl-acyl carrier protein desaturase gene from cotton); ssIIb (starch synthase IIb gene from maize); tNOS (terminator of the nopaline synthase gene); Zein (Zein gene from maize).