The Absence of N-Acetyl-D-glucosamine Causes Attenuation of Virulence of Candida albicans upon Interaction with Vaginal Epithelial Cells In Vitro

Figure 4

QRT-PCR analysis of the expression of GlcNAc catabolism genes. The relative gene expression of selected genes shows altered expression upon hyphae development as compared to yeast-like growth. First column (black bars) represents C. albicans cells grown in CKM (control hyphae); the second (open) and third (gray) columns stand for hyphae developed in the presence of PK E6/E7 vaginal epithelial cells and C. albicans cells grown in CKM + 10 mM of GlcNAc, respectively. The ratio of each mRNA relative to the 18S rRNA was calculated using the method. Data are representative of 3 independent experiments and are presented as mean ± standard deviation (SD). The significance of differences between sets of data was determined by two-sample -test; .