Hematopoietic Stem and Progenitor Cells Can Be Enriched by Implanting Biomaterial into Spatium Intermusculare

Figure 3

Hematopoietic stem and progenitor cells (HSPCs) in the migrating cells (MCs) were capable of long-term reconstitution in hematopoiesis in recipient mice. (a) Experimental scheme. After irradiation, the mice underwent transplantation with PBS (control), 1 × 10⁷ of male-derived MCs, or 1 × 10⁷ male-derived bone marrow (BM) cells. At 16 weeks after transplantation, BM, muscle, and spleen were sampled. (b) HSPCs in the MCs could home to the BM, muscle, and spleen of irradiated recipients. DNA samples were extracted, respectively, from the BM, muscle, and spleen, and male-derived cells were evaluated by PCR. Lane 1: female, Lanes 2–4: BM in control, MCs, and BM group, Lanes 5–7: muscle in control, MCs, and BM group, Lanes 8–10: spleen in control, MCs, and BM group, Lane 11: male, and Lane 12: marker. (c) Colony-forming unit (CFU) assay of BM cells derived from MCs transplantation recipients. Representative colonies were shown. Burst-forming unit-erythroid: BFU-E, colony-forming-unit-macrophage: CFU-M, colony-forming-unit-granulocyte, macrophage: GFU-GM, and colony-forming-unit-granulocyte, erythroid, macrophage, megakaryocyte: CFU-GEMM. (d) The percentage of individual Y-positive colonies in BM after MCs or BM transplantation was shown. The bars represented mean ± SD (); versus BM. (e) The survival numbers of mice in control, MCs, or BM transplantation.

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