Research Article
Prevention of Polyglycolic Acid-Induced Peritoneal Adhesions Using Alginate in a Rat Model
Table 8
(a) Immunohistochemical features on HBME-1 staining of mesothelial cells in Experiment 1. (b) Immunohistochemical features of CD68, CD86, and CD163 staining in three locations in Experiment 1.
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HBME-1 stained the single cell layer covering the tissue surface facing the peritoneal cavity. The HBME-1 staining was scored as follows: +++: clearly stained; ++: stained; and +: unclear. The HBME-1 staining was poor on the surface over the lymph follicles in the fibrin group and over the PGA fibers in the PGA alone group. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
(b) | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The macrophages were stained for CD68, CD86, and CD163 in three locations (1–3). The staining was described as follows: : mostly positive cells; : some positive cells; and ×: no positive cells. The visually recognized positive cells were scored as follows: +++: high; ++: medium; +: small; ±: few. (1) Cells between the fibers in the PGA bundles. (2) Cells in the space between the PGA bundles. (3) Cells on the surface of the PGA fiber. |