DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

Figure 1

Long DNA base synthesis protocol. Shorter fragments are synthesized chemically; then during hybridization with a slowly decreasing temperature, they form the proper molecule. Next, ligation joins the parts into a longer molecule. Finally, PCR with specific primers amplifies the correct DNA strands. If a correct molecule cannot be created because the fragments fold in an abnormal way, the reaction is performed in separate tubes (complex protocol).