Research Article
DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature
Table 1
Fragments used to synthesize the Ubp4′ gene; Ubp4′1–Ubp4′11 are used in the assembly protocol and Ubp4′For and Ubp4′Rev for PCR.
| Name | Sequence |
| Ubp4′1 | ATGTGCTACATGAATTGTATAATACAGTGTATCCTAGGAA | Ubp4′2 | AATCTGGGTCAGCTCATGCGTTCCTAGGATACACTGTATT | Ubp4′3 | CGCATGAGCTGACCCAGATTTTTCTCGATGACTCCTACGCTA | Ubp4′4 | TAGAGTTAATATTGATGTGCTTAGCGTAGGAGTCATCGAGA | Ubp4′5 | AGCACATCAATATTAACTCTAAACTAGGCTCTAAGGGCATCTTGG | Ubp4′6 | GCACGAGTCGAGCGAAATACTTTGCCAAGATGCCCTTAGAGCCT | Ubp4′7 | CAAAGTATTTCGCTCGACTCGTGCACATGATGTATAAAGAGCAAGTA | Ubp4′8 | GGGGAAATGGATATTTTCTTAGAACCGTCTACTTGCTCTTTATACATCAT | Ubp4′9 | GACGGTTCTAAGAAAATATCCATTTCCCCAATAAAATTTAAATTAGCATGTGGATCCGT | Ubp4′10 | GCGGTTTTGAACAACGAGTTTACGGATCCACATGCTAATTTAA | Ubp4′11 | AAACTCGTTGTTCAAAACCGCCTCACAACAGGATTGTCAGTAA |
| Ubp4′For | GGGGGAATTCGATATGTGCTACATGAATTGTATAATAC′ | Ubp4′Rev | GGGGGGATCCTTACTGACAATCCTGTTGTGAG |
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