DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

Table 1

Fragments used to synthesize the Ubp4^′ gene; Ubp4^′1–Ubp4^′11 are used in the assembly protocol and Ubp4^′For and Ubp4^′Rev for PCR.

Name	Sequence
Ubp4^′1	ATGTGCTACATGAATTGTATAATACAGTGTATCCTAGGAA
Ubp4^′2	AATCTGGGTCAGCTCATGCGTTCCTAGGATACACTGTATT
Ubp4^′3	CGCATGAGCTGACCCAGATTTTTCTCGATGACTCCTACGCTA
Ubp4^′4	TAGAGTTAATATTGATGTGCTTAGCGTAGGAGTCATCGAGA
Ubp4^′5	AGCACATCAATATTAACTCTAAACTAGGCTCTAAGGGCATCTTGG
Ubp4^′6	GCACGAGTCGAGCGAAATACTTTGCCAAGATGCCCTTAGAGCCT
Ubp4^′7	CAAAGTATTTCGCTCGACTCGTGCACATGATGTATAAAGAGCAAGTA
Ubp4^′8	GGGGAAATGGATATTTTCTTAGAACCGTCTACTTGCTCTTTATACATCAT
Ubp4^′9	GACGGTTCTAAGAAAATATCCATTTCCCCAATAAAATTTAAATTAGCATGTGGATCCGT
Ubp4^′10	GCGGTTTTGAACAACGAGTTTACGGATCCACATGCTAATTTAA
Ubp4^′11	AAACTCGTTGTTCAAAACCGCCTCACAACAGGATTGTCAGTAA
Ubp4^′For	GGGGGAATTCGATATGTGCTACATGAATTGTATAATAC^′
Ubp4^′Rev	GGGGGGATCCTTACTGACAATCCTGTTGTGAG