Research Article
DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature
Table 2
Fragments used to synthesize the Ubp4′′a gene; Ubp4′′a1–Ubp4′′a15 are used in the assembly protocol and Ubp4′′aFor and Ubp4′′aRev for PCR.
| Ubp4′′a1 | TTCGCGGTGGGCCTCGAGAATCTAGGAAATTCCTGCTATATGAACTGCATTATCC | Ubp4′′a2 | CAGCTCATGCGTCCCTAAAATACACTGGATAATGCAGTTCATATAGCAGGAATTTCCTAG | Ubp4′′a3 | AGTGTATTTTAGGGACGCATGAGCTGACGCAGATCTTCCTTGACGATTCGTATGCGAA | Ubp4′′a4 | CCCTAGTTTAGAATTGATATTGATGTGCTTCGCATACGAATCGTCAAGGAAGA | Ubp4′′a5 | GCACATCAATATCAATTCTAAACTAGGGTCGAAAGGTATTTTAGCTAAATATTTCGCTCGTCTTGTAC | Ubp4′′a6 | TTGGAGCCATCTACCTGTTCCTTATACATCATATGTACAAGACGAGCGAAATATTTAGCT | Ubp4′′a7 | ATATGATGTATAAGGAACAGGTAGATGGCTCCAAAAAAATTTCGATTAGCCCAATCAAATTTAAGCT | Ubp4′′a8 | AACAGTGAGTTGACGGAACCACAGGCGAGCTTAAATTTGATTGGGCTAATCG | Ubp4′′a9 | CGCCTGTGGTTCCGTCAACTCACTGTTTAAAACAGCTTCACAGCAAGATTGTCAGGAATTTTGCC | Ubp4′′a10 | GATCTTCATGCAAACCGTCAAGCAAGAATTGGCAAAATTCCTGACAATCTTGCTGTGA | Ubp4′′a11 | AATTCTTGCTTGACGGTTTGCATGAAGATCTGAACCAGTGCGGTTCTAACCCC | Ubp4′′a12 | CCTCTTGGCTCAGTTCTTTCAACGGGGGGTTAGAACCGCACTGGTTCA | Ubp4′′a13 | CCGTTGAAAGAACTGAGCCAAGAGGCAGAAGCGAGGCGGGAAAAGCTGAGCCTC | Ubp4′′a14 | GTTCCCATTCAATACTACTGGCTATGCGGAGGCTCAGCTTTTCCCGCCTCGC | Ubp4′′a15 | CGCATAGCCAGTAGTATTGAATGGGAACGCTTTCTGACCACGGATTTTAGCGTCATTGTG | Ubp4′′a16 | AGCGAGAGGCATATTGCCCTTGAAAGAGGTCCACAATGACGCTAAAATCCGTGG | Ubp4′′a17 | GACCTCTTTCAAGGGCAATATGCCTCTCGCTTAAAATGCAAAGTATGCAGCCACACTTCA |
| Ubp4′′aFor | GGGGGAATTCGATATGTTCGCGGTGGGCCTCGAGAATC | Ubp4′′bRev | GGGGGGATCCTTATGAAGTGTGGCTGCATACTTTGCATTTTAAG |
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