DNASynth: A Computer Program for Assembly of Artificial Gene Parts in Decreasing Temperature

Table 3

Fragments used to synthesize the Ubp4^′′b gene; Ubp4^′′b1–Ubp4^′′b17 are used in the assembly protocol and Ubp4^′′bFor and Ubp4^′′bRev for PCR.

Ubp4^′′b1	ACCACATATCAGCCCTTTACGGTACTGTCTATCCCTATACCCAAAAAGAATA
Ubp4^′′b2	AGCAATCCTCGATAGTAATGTTATTGCGACTATTCTTTTTGGGTATAGGGATAGACAGT
Ubp4^′′b3	GTCGCAATAACATTACTATCGAGGATTGCTTCCGCGAATTCACCAAATGCGAAAATCTCGAGGTAG
Ubp4^′′b4	TTCACAGTGCGGACAGAGCCATTGCTCATCTACCTCGAGATTTTCGCATTTGGT
Ubp4^′′b5	ATGAGCAATGGCTCTGTCCGCACTGTGAAAAACGTCAACCGTCAACTAAACAGCTGACCATTACC
Ubp4^′′b6	TTTTAGATGGACAATGAGGTTCCGCGGCAAGCGGGTAATGGTCAGCTGTTTAGTTGACGGT
Ubp4^′′b7	CGCTTGCCGCGGAACCTCATTGTCCATCTAAAACGTTTTGATAACCTGCTTAACAAAAACA
Ubp4^′′b8	AGCAGAAATGGATAAATCACGAAGTCATTGTTTTTGTTAAGCAGGTTATCA
Ubp4^′′b9	ATGACTTCGTGATTTATCCATTTCTGCTGGATTTAACGCCTTTTTGGGCAAACGACTT
Ubp4^′′b10	TTCACCCCCGGCGGGAACACGCCATCAAAGTCGTTTGCCCAAAAAGGCGTTA
Ubp4^′′b11	TGATGGCGTGTTCCCGCCGGGGGTGAACGACGATGAATTACCAATCCGAGGTCAGATCCCGCC
Ubp4^′′b12	CACGCGACACCATACAATTCATATTTAAATGGCGGGATCTGACCTCGGATTGGTAATT
Ubp4^′′b13	ATTTAAATATGAATTGTATGGTGTCGCGTGCCATTTCGGGACACTGTATGGCGGCCAC
Ubp4^′′b14	TTAAACCTTTTTTCACATAAGCCGTATAGTGGCCGCCATACAGTGTCCC
Ubp4^′′b15	TATACGGCTTATGTGAAAAAAGGTTTAAAAAAGGGATGGCTTTATTTTGATGA
Ubp4^′′b16	GCGTCGGCTTTGTTTTTAACCGGCTTATACTTGGTATCATCAAAATAAAGCCATCC
Ubp4^′′b17	TACCAAGTATAAGCCGGTTAAAAACAAAGCCGACGCAATTAATAGCAATGCATATGTTCTGTTCTAT
Ubp4^′′bFor	GGGGGGATCCACCACATATCAGCCCTTTACGGTAC
Ubp4^′′bRev	GGGGTCTAGATTAATGGTGATGGTGATGGTGATAGAACAGAACATATGCATTGCTATTAATTGCG