Black Rice Anthocyanins Suppress Metastasis of Breast Cancer Cells by Targeting RAS/RAF/MAPK Pathway

Figure 5

Black rice anthocyanins (BRACs) extract combined with raf-, mek-, or jnk-small interfering RNAs (siRNA) decreased mRNA expression of respective genes in MDA-MB-453 cells. MDA-MB-453 cells were treated with BRACs (0 or 200 μg/mL) with or without raf-, mek-, or jnk-siRNAs for 24 h. mRNA expression of (a) raf, (b) mek, (c) jnk was measured using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). ; data are mean ± SEM of three independent experiments.

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