Selection of Single-Stranded DNA Molecular Recognition Elements against Exotoxin A Using a Novel Decoy-SELEX Method and Sensitive Detection of Exotoxin A in Human Serum

Figure 1

Illustration of the Decoy-SELEX process. In vitro selection begins with incubation of target Exotoxin A with a library of 10¹⁵ ssDNA molecules. Binding molecules are amplified and subjected to incubation with multiple negative targets. Molecules that do not bind to negative targets are amplified and carried on to the next round of selection.