Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications

Figure 1

Illustration of the SELEX process. A random library consisting of up to 10¹⁵ single-stranded nucleic acids molecules are incubated with the target of interest. Those that bound to the target are retrieved and amplified by polymerase case reaction. It is then followed by incubation with negative targets. Those that do not bind to negative targets are retained, amplified, and subjected to further rounds of in vitro selection.