Review Article
Single-Stranded DNA Aptamers against Pathogens and Toxins: Identification and Biosensing Applications
Table 3
Summary table of ssDNA MREs targeting viruses and virus proteins for therapeutic applications.
| Virus | Target | SELEX method | | Reference |
| HIV | Reverse transcriptase | Nitrocellulose filters | — | [126] |
| HIV | Reverse transcriptase | Sephadex columns | 660 pM | [127] |
| HIV | Reverse transcriptase | Nitrocellulose filters | 1 nM | [128] |
| HIV | Reverse transcriptase | Primer-free SELEX | 82 nM | [129] |
| HIV | Integrase | Nitrocellulose filters | — | [130] |
| HIV | Integrase | — | — | [131] |
| HIV | Trans-activation-responsive RNA (TAR) element | Magnetic beads | 20 nM | [132] |
| HIV | Trans-activation-responsive RNA (TAR) element | Magnetic beads | 50 nM | [133] |
| HCV | NS5B RNA polymerase | Nitrocellulose filters | 132 nM | [134] |
| HBV | Core protein | — | High affinity determined by dot blot | [135] |
| SARS coronavirus | Helicase | Magnetic beads | 5 nM | [136] |
| Influenza A virus | Hemagglutinin from H3N2 | Ni-NTA beads | — | [137] |
| Influenza A virus | Hemagglutinin from H5N1 | Ni-NTA beads | High affinity determined by ELISA | [138] |
| Influenza A virus | Hemagglutinin from H3N2 | Ni-NTA magnetic beads | 7 nM | [139] |
| Influenza A virus | Hemagglutinin from H9N2 | Nitrocellulose filters | High affinity determined by ELISA | [140] |
| Influenza A virus | Nonstructural protein 1 | Glutathione agarose beads | 18.91 ± 3.95 nM | [141] |
| Rabies virus | Rabies virus infected BHK-21 cells | Cell-SELEX | 28 nM | [142] |
| HPV | HPV transformed HeLa cells | Cell-SELEX | 1 nM | [143] |
| Vaccinia virus | Whole virus particle | One-step MonoLEX | High affinity determined by dot blot, SPR, and so forth | [144] |
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