Dengue Patients Exhibit Higher Levels of PrM and E Antibodies Than Their Asymptomatic Counterparts

Figure 2

Restriction enzyme digestion profile of the recombinant plasmid DNA. Digestion of the recombinant clone produced a vector band and prM insert band at 508 bp. Lane M1: GeneRuler 1 kb plus DNA ladder marker (Fermentas); Lane L: linearized pET28b(+) plasmid with BamHI digestion with expected size 5368 bp; Lanes 1–6 are positive clones selected randomly out of 42 positive clones obtained. Shown here are the vectors at 5343 bp and the prM insert at 508 bp upon HindIII and BamHI digestion. Lane M2: 100 bp DNA ladder marker (Fermentas).