Effect of Recombinant Prophenin 2 on the Integrity and Viability of Trichomonas vaginalis

Figure 2

Electrophoretic analysis of pro-prophenin 2 expression, purification, and cleavage with hydroxylamine. Twelve percent polyacrylamide gels (20 : 1 acrylamide : bis-acrylamide) were used and proteins were stained with Coomassie blue. (a) Pro-prophenin 2 expression: lane 1, crude extract from E. coli BL21 STAR induced 4 h with 0.25 mM IPTG; lane 2, crude extract from uninduced E. coli BL21 STAR, otherwise grown as induced. (b) Pro-prophenin 2 purified by affinity chromatography (lane 1). (c) Processed prophenin 2 after hydroxylamine cleavage and purification (lane 1). M: molecular weight markers.

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