Effect of Recombinant Prophenin 2 on the Integrity and Viability of Trichomonas vaginalis

Figure 6

Stability of pro-prophenin 2 after interaction with T. vaginalis. (a) After parasite-peptide interaction at several incubation times, contents of wells (cells + supernatant) were analyzed by Western blot using anti-FLAG M2 antibodies and signal developed with luminol. Lane M: molecular weight markers; lane (+): 5 μM pro-prophenin 2 as a positive control. Lanes 1 to 3: T. vaginalis with 5 μM pro-prophenin 2 incubated 1, 2, and 4 h, respectively. Lanes 4 to 6: same as lanes 1 to 3 but in the presence of 10 μM E64. (b) Signal intensity analysis is done with Image Lab software (BioRad).

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