LAP TGF-Beta Subset of CD4⁺CD25⁺CD127⁻ Treg Cells is Increased and Overexpresses LAP TGF-Beta in Lung Adenocarcinoma Patients

Figure 2

Percentages of Th17 cells were identified according the membrane expression of ROR-γt or intracellular IL-17 in lung adenocarcinoma patients and smoking and nonsmoking control subjects. (a) Distribution of stimulated PBMCs according to forward scatter (FSC) and sideward scatter (SSC) dot plot. A gating was set for CD4⁺ T-cells. From gated CD4⁺ T-cells, cells producing IL-17, IFN-γ, or both cytokines were detected. A representative cytometric analysis from a lung adenocarcinoma patient is shown. (b) Percentages and expression levels (measure by iMFI values) of IL-17 from CD4⁺IL-17⁺ (Th17) T-cells are shown and compared among the studied groups. The results are reported as mean ± SEM. (c) Distribution of purified CD4⁺ T-cell in a FSC and SSC dot plot. A gating was set for CD4⁺ T-cells. From gated CD4⁺ T-cells, the percentage of ROR-γt⁺ cells was detected. A representative cytometric analysis from a lung adenocarcinoma patient is shown. (d) Percentages of CD4⁺ROR-γt⁺ T-cells from lung adenocarcinoma patients and smoking and nonsmoking control subjects are shown. The results are reported as mean ± SEM.

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