LAP TGF-Beta Subset of CD4⁺CD25⁺CD127⁻ Treg Cells is Increased and Overexpresses LAP TGF-Beta in Lung Adenocarcinoma Patients

Figure 3

Percentages of Treg cells phenotyped as CD4⁺CD25⁺CD127⁻ and percentages and expression levels of LAP TGF-β in Treg cells from lung adenocarcinoma patients and smoking and nonsmoking subjects. (a) Distribution of purified CD4⁺ T-cell in a forward scatter (FSC) and sideward scatter (SSC) dot plot. A gating was set for CD4⁺ T-cells. From gated CD4⁺ T-cells, the percentage of CD25⁺CD127⁻ cells was detected. A representative cytometric analysis from a lung adenocarcinoma patient is shown. (b) Percentages of Treg cells from lung adenocarcinoma patients and smoking and nonsmoking control subjects are shown. The results are reported as mean ± SEM. (c) The histograms indicate the percentages of gated unstimulated Treg cells expressing FOXP3 or CTLA-4. From stimulated CD4⁺ T-cells, the percentage of LAP TGF-β⁺ cells from Treg cells was detected. A representative cytometric analysis from a lung adenocarcinoma patient is shown. (d) Percentages and expression levels (measure by MFI and iMFI values) of LAP TGF-β from CD4⁺CD25⁺CD127⁻ T-cells are shown and compared among the studied groups. The results are reported as mean ± SEM.

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