Preclinical Evaluation of the Immunomodulatory Properties of Cardiac Adipose Tissue Progenitor Cells Using Umbilical Cord Blood Mesenchymal Stem Cells: A Direct Comparative Study

Figure 2

T cell alloproliferation induced by MDDCs is abrogated with either cardiac ATDPCs or UCBMSCs. Proliferation was calculated as % of FSCFS T cells after a 4.5-day coculture with allogeneic mature MDDCs in 20 : 1 and 40 : 1 ratios, together with different numbers (1,250, 2,500, 5,000, 10,000, and 20,000 cells) of either cardiac ATDPCs (a) or UCBMSCs (b). Viability of T cells was assessed according to FSC/SSC gating in coculture with cardiac ATDPCs (c) and UCBMSCs (d). Results are reported as % of proliferation or viability relative to the proliferation or viability level of T cells stimulated with MDDCs in a 20 : 1 ratio, respectively. Data is depicted as mean ± SD of four independent experiments ( for UCBMSCs, cardiac ATDPCs, and responder T cells), calculated from three experimental replicates. Statistical differences, indicated as and , are only shown for the 20 : 1 T cell : MDDC ratio.

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