Inhibition of Adhesion, Proliferation, and Invasion of Primary Endometriosis and Endometrial Stromal and Ovarian Carcinoma Cells by a Nonhyaluronan Adhesion Barrier Gel

Figure 2

Microscopic analysis on day 6 showing inhibition of cellular adherence, proliferation, and invasion in the presence (+) or absence (−) of a barrier gel. (a) Left panels show schematic drawings of the overall cell culture experimental design and outcome of results. Top microscope pictures show normal mitotic growth of Ec-ESC without the presence of a barrier substance with the boxed region representing a magnification (right picture). Bottom microscopic pictures show a region, where cells were inhibited in adherence when the barrier gel was entirely coated on the bottom of a 35 mm tissue culture well. The boxed region shows a magnification (right picture). Note that Ec-ESC cells are rounded and floating. The presence of the barrier gel also led to lower cellular proliferation as represented in the graph to the right. Graph shows percentage of cellular growth after 6 days of culture of Eu-ESC and Ec-ESC and both ovarian carcinoma cell lines, when the barrier substance was entirely coated on the bottom of a 35 mm cell culture well. Untreated cells were set to 100%; mean values (numbers above graphs) are shown from experiments of Eu-ESC and Ec-ESC (triplicates) as well as ovarian carcinoma cell lines (duplicates). Percentage values in the presence of the barrier gel were for Eu-ESC: 55.9 +/− 0.67; Ec-ESC: 53.4 +/− 4.2; TOV-21G: 17 +/− 4.0; TOV112D: 54 +/− 2.0. (b) Left panels show schematic drawings of the overall cell invasion experimental design and outcome of results. Top microscope pictures show the collagen surface without a barrier substance and Ec-ESC adhered cells, which invaded collagen. The right picture represents a magnification of the left box. The arrows indicate Ec-ESC, which invaded below the collagen surface. The bottom panel represents the results of Ec-ESC in the presence of a barrier gel entirely coated over the collagen surface. A region is shown, where Ec-ESC cells were inhibited in adherence and invasion and thus were rounded and floating in media above the collagen surface. The boxed region shows a magnification (right picture). Right two graphs show the amount of invaded cells into collagen per cm² after 6 days with or without the barrier gel coated on the collagen surface. Mean values of invaded cells are shown in the graphs in the presence (+) or absence (−) of the barrier gel: Eu-ESC: 72 +/− 5.6 (−); Eu-ESC: 17 +/− 6.3 (+); Ec-ESC: 760 +/− 40.7 (−); Ec-ESC: 268 +/− 14.6 (+); TOV-21G: 2,223 +/− 74.2 (−); TOV-21G: 0 +/− 0.0 (+); TOV112D: 3,287 +/− 389.9 (−); TOV112D: 70 +/− 11.2 (+).

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