Inhibition of Adhesion, Proliferation, and Invasion of Primary Endometriosis and Endometrial Stromal and Ovarian Carcinoma Cells by a Nonhyaluronan Adhesion Barrier Gel

Figure 3

Inhibition of TOV-21G cellular invasion into a collagen matrix on day 6. (a) Top left picture shows a microscopic region focused on the collagen surface on day 6. Cells adhered to the surface are in focus (e.g., arrowhead), whereas the out of focus cells have invaded to different depths beneath the surface (arrows). After focusing the microscope beneath the surface of the collagen, the deepest invaded cell from the top picture is now visible (middle arrow and bottom left picture), whereas the other two cells are out of focus (arrowhead and arrow). Two right pictures represent a collagen surface entirely coated with the barrier gel on day 6 of cell culture. Top right picture shows a region, where no cells have adhered to the collagen surface. Arrows point to two out of focus floating cells. After focusing the microscope above the collagen surface in the media both dead cells became visible (arrows and bottom right picture).

(a)

(b)

(c)

(d)