miR-136 Modulates TGF-β1-Induced Proliferation Arrest by Targeting PPP2R2A in Keratinocytes

Figure 4

PPP2R2A was involved in TGF-β1-induced proliferation arrest in HaCaT cells. (a) Western blot analysis of PPP2R2A expression in response to TGF-β1 treatment. GAPDH was used as internal control. (b) Three independent results of Western blot were quantified by Image J. (c) PPP2R2A silencing was verified by quantitative real-time PCR. (d) Determination of cell viability with Cell Counting Kit-8. (e) Flow cytometry analysis of the cell cycle. Bars indicate SD. .

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