Modifications in Bone Matrix of Estrogen-Deficient Rats Treated with Intermittent PTH

Figure 2

Increased bone mass in rats treated with low doses of intermittent PTH. (a) Photo from whole femur. The black box indicates the region of interest (ROI) in the distal femora that histomorphometry, histochemistry, TUNEL assay, and GAGs quantification were performed. Observe a light micrograph from bone section stained with hematoxylin and eosin (H&E) showing the region of interest for histomorphometric analysis (black square). An area of 3 mm² was evaluated at 390 μm below the lowest point of the growth plate and 390 μm from the outer cortical surface. To measure cortical thickness, the average from three cortical thicknesses per section was calculated in the metaphyseal region, using the mean of three measurements from the same box used to measure the cancellous area. Ct: cortical; Ma: bone marrow; Tb: trabecula; Gp: growth plate. Scale bar represents 200 μm. (b) Cancellous bone volume (BV/TV) and cortical thickness (Ct.Wi) were measured by histomorphometry in the ROI of estrogen-deficient rats treated with vehicle or at indicated doses of iPTH. (c) Tibial BMD was measured in whole tibia by Archimedes principle. Data are expressed as mean ± SD. –11. by one-way ANOVA.

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