Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS

Figure 1

Study design and workflow of prostate cancer biomarker candidate identification (a) and validation (b). (a) Prostate cancer tissue from patients with and without recurrence as well as tumor-free tissue was analyzed using two-dimensional differences in gel electrophoresis (2D-DIGE) and mass spectrometry (MS). (b) Identified potential new biomarker candidates were validated using Western blots, immunohistochemistry, tissue microarrays (TMA), and multiple reaction monitoring (MRM).

(a)

(b)