Research Article
Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS
Table 2
Antibodies used for Western blot analysis.
| | Antibody | Host | Type | Company | Dilution |
| | β-Actin (A5441) | Mouse | Monoclonal | Sigma Aldrich, St. Louis, USA | 1 : 500 | | Secernin-1 | Rabbit | Polyclonal | Sigma Aldrich, St. Louis, USA | 1 : 500 | | Vinculin | Mouse | Monoclonal | Fitzgerald, North Acton, USA | 1 : 1,000 | | Anti-mouse + HRP (P0447) | Goat | Polyclonal | DAKO, Hamburg, Germany | 1 : 5,000 | | Anti-rabbit + HRP (P0448) | Goat | Polyclonal | DAKO, Hamburg, Germany | 1 : 5,000 | | Peroxidase anti-Mouse IgG (PI-2000) | Horse | Polyclonal | Vector Laboratories, USA | 1 : 10,000 | | Peroxidase anti-Rabbit IgG (PI-1000) | Goat | Polyclonal | Vector Laboratories, USA | 1 : 20,000 |
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