Identification and Validation of Potential New Biomarkers for Prostate Cancer Diagnosis and Prognosis Using 2D-DIGE and MS
Table 5
Deregulated proteins in recurrent prostate cancer identified with 2D-DIGE and MS.
Spot
Acc. no.
Protein name
Ratio + versus − rec
value (-test) + versus − rec
value (-test) + versus − rec
Down 13
P01857
Ig gamma-1 chain C region
−274.3
0.334
0.041
Down 01
FLNA
Filamin-A
−12.0
0.269
0.031
Down 12
SCRN1
Secernin-1
−8.9
0.088
0.044
Down 04
O95394
Phosphoacetylglucosamine mutase
−7.5
0.228
0.012
Down 08
PYGB
Glycogen phosphorylase, brain form
−5.6
0.176
0.031
Down 03
P06396
Gelsolin
−5.3
0.092
0.023
Down 02
P06396
Gelsolin
−3.5
0.109
0.019
Down 16
LEG3
Galectin-3
−3.4
0.272
0.033
Down 05
PTGR2
Prostaglandin reductase 2
−3.2
0.047
1.169
Down 06
LMNA
Lamin-A/C
−2.6
0.036
0.023
Down 14
TTC38
Tetratricopeptide repeat protein 38
−2.4
0.087
0.036
Down 15
Mix
MDHM
Malate dehydrogenase, mitochondrial
−2.3
0.233
0.014
P51911
Calponin-1
−2.3
0.233
0.014
Down 09
P16870
Carboxypeptidase E precursor
−2.2
0.122
0.049
Down 10
CO6A2
Collagen alpha-2(VI) chain
−1.9
0.018
0.031
Down 07
LMNA
Lamin-A/C
−1.7
0.133
0.036
Down 11
G6PD
Glucose-6-phosphate 1-dehydrogenase
−1.7
0.255
0.036
Up 08
Mix
Q9UBR2
Cathepsin Z precursor
1.2
0.529
0.951
P20774
Mimecan precursor
1.2
0.529
0.951
Up 10
CAZA1
F-actin-capping protein subunit alpha-1
1.5
0.209
0.042
Up 05
PAPP
Prostatic acid phosphatase
1.8
0.048
0.056
Up 01
VINC
Vinculin
2.2
0.027
0.031
Up 03
GRP78
78 kDa glucose-regulated protein
2.5
0.025
0.036
Up 02
COEA1
Collagen alpha-1(XIV) chain
2.5
0.075
0.049
Up 09
KCD12
BTB/POZ domain-containing protein KCTD12
2.6
0.158
0.045
Up 11
GLO2
Hydroxyacylglutathione hydrolase, mitochondrial
3.2
0.047
0.042
Up 12
Mix
ANXA5
Annexin A5
3.2
0.032
0.074
A1BG
Alpha-1B-glycoprotein
3.2
0.032
0.074
Up 04
SYNEM
Synemin
4.9
0.055
0.027
Up 13
PRDX4
Peroxiredoxin-4
4.9
0.022
0.004
Up 06
ANXA4
Annexin A4
6.4
0.288
0.042
Up 07
TCPA
T-complex protein 1 subunit alpha
51.9
0.275
0.031
2D-DIGE: two-dimensional differences in gel electrophoresis; MS: mass spectrometry; Acc. no.: accession number; + rec: prostate cancer with recurrence; − rec: prostate cancer without recurrence; -Test: two-sided Mann-Whitney -Test; ratio: division of the mean; mean: normalized spot volume.
