PTEN Plays an Important Role in Thrombin-Mediated Lung Cancer Cell Functions

Figure 2

The effects of thrombin on PTEN expression and the PI3K/AKT signaling pathway. Glc-82 cells were starved for 24 h in serum-free medium before treatment with thrombin (0.5 U/mL) for 8 h, with or without LY-294002 (50 μM). Total protein extracts were analyzed for the protein levels of PTEN (a), p-AKT (b), Skp2 (c), and p27 (d) by western blotting. (e) Effect of the PI3K inhibitor LY-294002 and thrombin on PTEN protein expression. (f) Effect of LY-294002 and thrombin on p-AKT. GAPDH and AKT were analyzed as internal loading controls. and , compared with thrombin (0 U/mL).

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