PTEN Plays an Important Role in Thrombin-Mediated Lung Cancer Cell Functions

Figure 3

PTEN plays a role in thrombin-mediated activation of the PI3K/AKT signaling pathway. Glc-82, Mock, and Glc-PTEN-shRNA cells were starved for 24 h in serum-free medium before treatment without or with thrombin (0.5 U/mL) for 8 h. The protein levels of PTEN (a), p-AKT (b), Skp2 (c), and p27 (d) were analyzed by western blotting. (e) An anti-PAR1 antibody (0.5 ng/mL) blocked thrombin-mediated PTEN downregulation. (f) The overexpression of PAR1 enhanced the decrease of PTEN expression mediated by thrombin. and , compared with thrombin (0 U/mL).

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