PTEN Plays an Important Role in Thrombin-Mediated Lung Cancer Cell Functions

Figure 4

The role of PTEN in thrombin-mediated effects on Glc-82 cells. (a) The role of PTEN in thrombin-mediated promotion of Glc-82 cell cycle progression. Glc-82, Mock, and Glc-PTEN-shRNA cells were starved for 24 h in serum-free medium before treatment without or with thrombin (0.5 U/mL) for 12 h. The cell cycle was evaluated by flow cytometer analysis. (b) The role of PTEN in thrombin-mediated protection of Glc-82 cells from serum deprivation-induced apoptosis. Cells were starved for 48 h in serum-free RPMI 1640 medium. Thrombin (0.5 U/mL) was added and incubated for a further 96 h. The cells were then collected and analyzed on the flow cytometer within 1 h after staining. (c) The role of PTEN in thrombin-mediated promotion of Glc-82 cells migration. Cells were allowed to migrate for 20 h, cells in serum-free medium without or with thrombin (0.5 U/mL); then three different visual fields were randomly selected to count the cell number and calculate the average migration rate (, ).

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