Cholesteryl Pullulan Encapsulated TNF-α Nanoparticles Are an Effective Mucosal Vaccine Adjuvant against Influenza Virus

Figure 5

Flow cytometry of NALT cells after immunization with TNF/CHP nanoparticles. Mice were nasally administered with IVV (0.3 μg/mouse) or IVV and TNF/CHP nanoparticles (5 μg/mouse as TNF-α) once a week for 3 times. Sixty-eight hours after the last immunization, NALT cells were prepared and subjected to flow cytometric analysis. The expression of CD11c and CD80/CD86 was examined. (a) and (d), saline; (b) and (e), IVV; (c) and (f), IVV with TNF/CHP nanoparticles; (a), (b), and (c), CD80⁺/CD11c⁺; and (d), (e), and (f), CD86⁺/CD11c⁺.

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