Cholesteryl Pullulan Encapsulated TNF-α Nanoparticles Are an Effective Mucosal Vaccine Adjuvant against Influenza Virus

Figure 6

Antigen uptake of NALT and nasal passage DCs after TNF/CHP nanoparticles administration. Mice were immunized for 6 h with 10 μg of Alexa-labeled OVA antigen for nasal administration with or without TNF/CHP nanoparticles as an adjuvant. The NALT and the nasal passage cells were prepared and subjected to FCM analysis with Gatelogic software and antigen uptake was measured by the Alexa 647 florescence intensity for DCs (CD11c⁺ cells) detected in parallel, that is, ratio of Alexa 647⁺/CD11c⁺. (a) NALT DCs, (b) nasal passage DCs. MFI, mean fluorescence intensity (mean ± SD, ).

(a)

(b)