A Tissue Retrieval and Postharvest Processing Regimen for Rodent Reproductive Tissues Compatible with Long-Term Storage on the International Space Station and Postflight Biospecimen Sharing Program

Table 2

Percent total motility (±SD) of mouse cauda epididymal sperm after exposure of epididymis to various posteuthanasia conditions.

Time after euthanasia	In carcass	On ice	At room temperature
0 h	48 ± 18	49 ± 29	45 ± 21
0.5 h	51 ± 12	47 ± 11	50 ± 15
1.0 h	53 ± 6	60 ± 21	40 ± 9
1.5 h	57 ± 12	67 ± 16	54 ± 11
2.0 h	51 ± 14	46 ± 14	43 ± 14
2.5 h	50 ± 17	49 ± 18	52 ± 12

Tissue was submerged in Ham’s F-10 medium in a 15 mL culture tube which was placed on ice or at room temperature as indicated. Values are mean ± standard deviation ( mice at each time point). There was no significant difference in motility between the three testing conditions at each time point.